Influence of HgCl2 on the expression of fibrillarin (Fb) in germination of Phaseolus vulgaris LFibrilarina
Keywords:
Fibrilarin, Mercuric chloride, Western blot, proteins, germinationAbstract
Mercury (Hg) is now considered an environmental contaminant of high risk to human health and the environment. The toxicity of mercury and its compounds has been extensively documented, but the molecular mechanisms of tox- icity have not been fully clarified. The aim of this study was to analyze the effect of mercuric chloride (HgCl2) in the expression of Fb, as a biomarker of early effect of mercury contamination, using as a model of Phaseolus vulgaris L. seeds, black variety Jamapa . Fibrillarin (Fb) one of the main components of the nucleolus, is an essential ribonucleo- protein phylogenetically conserved in ribosomal RNA processing (rRNA) in partnership with small nucleolar RNAs (snoRNAs), along with other basic functions in biological systems. The expression of Fb was monitored by protein electrophoresis (SDS-PAGE) and Western blot of embryos beans during early stages of germination with different times 0, 6, 12, 18, 24 and 30 hrs and exposure 10 , 20 and 30 µM HgCl2. The results obtained by Western blot re- vealed that inhibits mercury nucleolar protein expression Fb from a minimum concentration of 10 µM HgCl2 after 18 hours after the start of germination. These observations demonstrate that the protein is usually a mercury target mole- cule capable of inhibiting its expression. This particular selectivity of HgCl2 by protein may constitute the biochemi- cal basis for considering Fb as early biomarker of contamination by HgCl2 Additionally the results show that the inhi- bition HgCl2 and caused marked delay seed germination gradually, as the dose of mercury is increased to 30 µM HgCl2.
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